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Established A Index Adapters Consult with Index two (i5) Orientation To find out more regarding how to enter i5 bases within the sample sheet in forward or reverse complement orientation.
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If managing in handbook mode and employing bcl2fastq or BCL Transform, or uploading the sample sheet to BaseSpace as a guide method run and attaching a sample sheet, enter the i5 sequence from the ahead direction.
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NextSeq one thousand/2000 and NovaSeq X/X In addition i5 indexes will properly be output within the forward orientation for afterwards downstream processing. Tailor made kits also needs to hold the i5 indexes entered while in the forward orientation.
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Take a look at how AI is reworking genomic investigation, driving medical insights, and enabling multiomic discovery
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